CD68 抗体
(11 references)Quick Overview for CD68 抗体 (ABIN181837)
抗原
See all CD68 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This monoclonal FA-11 antibody does detect surface Macrosialin at low levels in resident Mouse peritoneal Macrophages which can be enhanced with thioglycollate stimulation.
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交叉反应 （详细）
- Species reactivity (tested):Mouse.
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纯化方法
- Affinity Chromatography on Protein G
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免疫原
- Purified Concanavalin A acceptor glycoprotein from P815 cell line.
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亚型
- IgG2a
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anti-CD68 Molecule (CD68) (AA 312-326), (Middle Region) antibody
CD68 适用: 小鼠, 大鼠 IHC, FACS, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-CD68 Molecule (CD68) (AA 42-155) antibodyCD68 适用: 人 IHC, FACS, WB, ELISA, ICC 宿主: 小鼠 Monoclonal 3F7D3 unconjugated
anti-CD68 Molecule (CD68) antibodyCD68 适用: 人, 小鼠, 猴, 大鼠 IHC, FACS, WB, IF, ISt, StM 宿主: 小鼠 Monoclonal C68-684 unconjugated
anti-CD68 Molecule (CD68) (AA 22-319) antibodyCD68 适用: 人, 小鼠, 大鼠 ELISA, IHC (p), ICC, IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-CD68 Molecule (CD68) antibodyCD68 适用: 人 IHC, FACS, WB, IF, ISt, StM 宿主: 小鼠 Monoclonal rLAMP4-824 unconjugated Recombinant Antibody
anti-CD68 Molecule (CD68) (AA 150-301) antibodyCD68 适用: 人 IHC, FACS, ELISA, ISt, StM, Coat 宿主: 小鼠 Monoclonal LAMP4-1830 unconjugated
anti-CD68 Molecule (CD68) (AA 22-319) antibodyCD68 适用: 人 IHC, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-CD68 Molecule (CD68) (AA 150-221) antibodyCD68 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal C68-2709 unconjugated
anti-CD68 Molecule (CD68) antibody (PE)CD68 适用: 人 FACS 宿主: 小鼠 Monoclonal Y1-82A PE
anti-CD68 Molecule (CD68) (AA 341-354), (C-Term) antibodyCD68 适用: 人 IHC, WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western Blot. Immunoprecipiation. Immunohistochemistry on frozen sections. Flow Cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL, Membrane permeabilisation is required for this application.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, pH 7.4, 0.09 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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: "MDA-5 activation by cytoplasmic double-stranded RNA impairs endothelial function and aggravates atherosclerosis." in: Journal of cellular and molecular medicine, Vol. 20, Issue 9, pp. 1696-705, (2017) (PubMed).
: "Myeloid-Specific Deletion of Diacylglycerol Lipase α Inhibits Atherogenesis in ApoE-Deficient Mice." in: PLoS ONE, Vol. 11, Issue 1, pp. e0146267, (2016) (PubMed).
: "Visualization of molecular fluorescence point spread functions via remote excitation switching fluorescence microscopy." in: Nature communications, Vol. 6, pp. 6287, (2015) (PubMed).
: "The Leishmania donovani histidine acid ecto-phosphatase LdMAcP: insight into its structure and function." in: The Biochemical journal, Vol. 467, Issue 3, pp. 473-86, (2015) (PubMed).
: "Successful use of mRNA-nucleofection for overexpression of interleukin-10 in murine monocytes/macrophages for anti-inflammatory therapy in a murine model of autoimmune myocarditis." in: Journal of the American Heart Association, Vol. 1, Issue 6, pp. e003293, (2013) (PubMed).
: "Knockdown of ICB-1 gene enhanced estrogen responsiveness of ovarian and breast cancer cells." in: Endocrine-related cancer, Vol. 17, Issue 1, pp. 147-57, (2010) (PubMed).
: "B cell-derived vascular endothelial growth factor A promotes lymphangiogenesis and high endothelial venule expansion in lymph nodes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 184, Issue 9, pp. 4819-26, (2010) (PubMed).
: "SIGNR1-negative red pulp macrophages protect against acute streptococcal sepsis after Leishmania donovani-induced loss of marginal zone macrophages." in: The American journal of pathology, Vol. 175, Issue 3, pp. 1107-15, (2009) (PubMed).
: "Human lymphocyte activation gene-3 molecules expressed by activated T cells deliver costimulation signal for dendritic cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 6, pp. 3782-8, (2008) (PubMed).
: "Activation of fractalkine/CX3CR1 by vascular endothelial cells induces angiogenesis through VEGF-A/KDR and reverses hindlimb ischaemia." in: Cardiovascular research, Vol. 78, Issue 2, pp. 333-40, (2008) (PubMed).
: "T cell activation-associated epitopes of CD147 in regulation of the T cell response, and their definition by antibody affinity and antigen density." in: International immunology, Vol. 11, Issue 5, pp. 777-86, (1999) (PubMed).
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: "MDA-5 activation by cytoplasmic double-stranded RNA impairs endothelial function and aggravates atherosclerosis." in: Journal of cellular and molecular medicine, Vol. 20, Issue 9, pp. 1696-705, (2017) (PubMed).
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- CD68 (CD68 Molecule (CD68))
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别名
- CD68
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背景
- The CD68 antigen is a 37kD transmembrane protein that is post-translationally glycosylated to give a protein of 87-115kD. CD68 is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells. Macrosialin is the murine homologue of the human macrophage glycoprotein CD68, both are members of the lysosomal-associated membrane protein (lamp) family which are located predominantly within the cells and can be detected by flow cytometry using cell permeablisation.Synonyms: Gp110, Macrophage marker, Macrosialin
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基因ID
- 12514
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NCBI登录号
- NP_033983
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UniProt
- P31996
抗原
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